In this study, the CIMFS-96 system developed by CELLAMES Inc. was used in combination with the Resipher system from Lucid Scientific, Inc. to measure changes in impedance and oxygen consumption rate (OCR) following cisplatin treatment in A549 cells. The relationship between OCR and impedance measurements was then evaluated.
Following treatment with cisplatin at different concentrations (0, 1, 10, and 100 μM), OCR and impedance showed similar cisplatin-induced response trends. However, because OCR primarily reflects mitochondrial oxidative metabolism whereas impedance reflects cell adhesion and morphology, the two measurements should be interpreted as complementary readouts rather than directly proportional parameters.
In addition, overall impedance profiles were comparable between conditions with and without Resipher-based OCR measurements, suggesting that the influence of Resipher measurements on impedance analysis was limited. Impedance measurements also reflected morphology-related changes observed in brightfield images, including changes in cell spreading and cell-electrode coverage, indicating that impedance analysis can provide additional information not directly captured by OCR data. OCR response profiles were similar between ITO electrode-based cell chips and SUS316L electrode-based PCB cell chips, suggesting that the differences in the electrode/substrate configurations had limited influence on OCR measurements under the tested conditions.
Taken together, these findings suggest that the combined use of OCR and impedance measurements may provide a complementary approach for evaluating drug-induced cellular responses.
The purpose of this experiment is to evaluate the dose-dependent cytotoxic effects of cisplatin on A549 cells by monitoring impedance changes and determining the IC50 value using the CIMS-32 system.
Cellames Cell Analysis TeamThe purpose of this experiment is to evaluate the suitability of different coating materials for supporting A549 cell attachment and proliferation in impedance-based assays.
Cellames Cell Analysis Team